INP

Leibniz Institute for Plasma Science and Technology
Felix-Hausdorff-Str. 2
17489 Greifswald
GERMANY

https://www.inp-greifswald.de/en/
welcomeatinp-greifswald [punkt] de

The Leibniz Institute for Plasma Science and Technology (INP) is the largest non-university institute in the field of low temperature plasmas, their basics and technical applications in Europe. The institute carries out research and development from idea to prototype. The topics focus on the needs of the market. At present, plasmas for materials and energy as well as for environment and health are the focus of interest.

License

Creative Commons Attribution 4.0 International (CC BY 4.0)
[Open Data]

Cite Dataset

Metadata Export

Bioimaging data management workflow for plasma medicine - dataset

Plasma Medicine

This dataset documents a complete, FAIR-aligned bioimage data workflow integrating Adamant, eLabFTW, and OMERO for an A375 melanoma cell imaging experiment. It includes stepwise visualizations of metadata collection, experiment documentation, data import, annotation, and image rendering across the three platforms. The dataset provides structured metadata at multiple levels, "screen", "plate", and "well", as well as instrument, captured using Adamant, eLabFTW, and Micro-Meta App. Comprehensive biological metadata for each well is supplied in CSV file. Detailed microscope-configuration metadata is provided as JSON files. All raw images are included in ".czi" format and corresponding "TIFF/XML" exports (images as .zip file). These materials enable users to test, reproduce, and validate the full workflow, from metadata acquisition and experiment documentation to data import, annotation, and image visualization, providing a FAIR reference implementation of the workflow.

research data management
FAIR
bioimaging
plasma medicine
FieldValue
Group
INP
Authors
Ahmadi, Mohsen
Wagner, Robert
Bekeschus, Sander
Becker, Markus M.
Release Date
2026-01-26
Identifier
6d14d866-9487-4688-84f3-7962a8b6c0ae
Permanent Identifier (DOI)
10.34711/inptdat.1013
Permanent Identifier (URI)
https://www.inptdat.de/node/1013
Plasma Source Name
kINPen® MED
Plasma Source Application
biomedical applications
Plasma Source Specification
AC
high frequency
atmospheric pressure
non-thermal
Plasma Source Properties

Power: < 3.5 W
Frequency: 1 MHz (radiofrequency-driven plasma jet)
Working gas: Argon
Gas flow rate: 1.5 standard liters per minute (slm)
Effluent temperature: 35–38 °C at the nozzle, ensuring tissue-compatible, non-thermal operation
Plasma Source Procedure

The device generates a stable cold plasma plume that delivers a mixture of reactive oxygen and nitrogen species (RONS) while maintaining a temperature safe for biological samples. More details are available in published literature, see https://doi.org/10.1088/1361-6463/aab3ad.
Plasma Medium Name
Ar
Plasma Medium Properties

Argon (Ar) as feed gas: The plasma was generated using argon in ambient air at 1 atm, with a pre-pressure of approximately 5 atm. The gas temperature during operation remained in the range of 20–25 °C.
Plasma Medium Procedure

The argon flow rate was regulated using an external mass flow controller (Bronkhorst, NL; model F-201 CM with controller E-8412-A-10, 5 slm range), even though the plasma device itself is equipped with an internal flow regulation unit.
Plasma Target Name
A375 cell line
Plasma Target Properties
Human epithelial skin cancer cells A375 cultured in 300 µL RPMI medium supplemented with Fetal Calf Serum (FCS), L-glutamine, penicillin, and streptomycin (50 µL of each additive).
Plasma Target Procedure
Human epithelial skin cancer cells A375 cultured in 300 µL RPMI medium supplemented with Fetal Calf Serum (FCS), L-glutamine, penicillin, and streptomycin (50 µL of each additive) were exposed to plasma . Cells were seeded in a 96-well NUNC Edge plate (Thermo Scientific) and maintained under standard conditions. Plasma exposure parameters varied by treatment duration and nozzle-to-surface distance. All treatments were performed on a motorized XYZ stage to provide controlled positioning at RT and ambient humidity.
Plasma Diagnostics Name
bioimaging
Plasma Diagnostics Properties

Axio Observer Z1 (Carl Zeiss) fluorescence microscope
Plasma Diagnostics Procedure

Cells were imaged using an Axio Observer Z1 (Carl Zeiss) fluorescence microscope, acquiring data in the DAPI channel. The imaging setup and associated parameters, including acquisition settings, objective specifications, and microscope configurations, were documented in detail. All relevant metadata from the microscope are available in JSON format (AxioZ1MicroscopeDescription.json). This metadata file contains information about the imaging conditions and setup. This metadata file is accessible for further analysis and integration with image processing workflows.
Language
English
License
Creative Commons Attribution 4.0 International (CC BY 4.0)
Public Access Level
Public
Contact Name
Ahmadi, Mohsen
Contact Email
mohsen.ahmadi@inp-greifswald.de

Data and Resources